Enzymatic vitreous disruption

Enzymatic breakdown of type II collagen in the human vitreous.

PURPOSE To investigate whether enzymatic collagen breakdown is an active process in the human vitreous. METHODS Human donor eyes were used for immunohistochemistry to detect the possible presence of the matrix metalloproteinase (MMP)-induced type II collagen breakdown product col2-3/4C-short in the vitreous. Western blot and slot blot analyses were used to further identify vitreal type II col...

Trypsin-mediated enzymatic degradation of type II collagen in the human vitreous

PURPOSE Aging of the vitreous body can result in sight-threatening pathology. One aspect of vitreous aging is liquefaction, which results from the vanishing of collagen fibrils. We investigated the possibility that trypsins are involved in vitreous type II collagen degradation. METHODS Immunohistochemistry and western blotting were used for detecting and locating trypsin isoforms in the vitre...

[Posterior vitreous detachment and pharmacologic vitreolysis: the new age of enzymatic vitrectomy].

Quenching of acyl-homoserine lactone-dependent quorum sensing by enzymatic disruption of signal molecules.

Many Gram-positive and Gram-negative bacteria communicate using small diffusible signal molecules called autoinducers. This process, known as quorum sensing (QS), links cell density to the expression of genes as diverse as those associated with virulence factors production of plant and animal pathogens, bioluminescence, antibiotic production, sporulation or biofilm formation. In Gram-negative b...

Combined Enzymatic and Mechanical Cell Disruption and Lipid Extraction of Green Alga Neochloris oleoabundans

Microalgal biodiesel is one of the most promising renewable fuels. The wet technique for lipids extraction has advantages over the dry method, such as energy-saving and shorter procedure. The cell disruption is a key factor in wet oil extraction to facilitate the intracellular oil release. Ultrasonication, high-pressure homogenization, enzymatic hydrolysis and the combination of enzymatic hydro...